DNA

Part:BBa_K4342014:Design

Designed by: Keaton Brown   Group: iGEM22_Austin_UTexas   (2022-10-04)


recJ Downstream


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 646
    Illegal BamHI site found at 874
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed to ligate to the tdk/kan cassette and the RecJ Upstream Homology via bsaI and bsmbI digestions.



Source

Acinetobacter baylyi ADP1


References